Effect of Antisperm Antibodies Present in Human Follicular Fluid upon the Acrosome Reaction and Sperm–Zona pellucida Interaction
Article first published online: 11 AUG 2003
American Journal of Reproductive Immunology
Volume 50, Issue 3, pages 209–219, September 2003
How to Cite
Marín-Briggiler, C. I., Vazquez-Levin, M. H., Gonzalez-Echeverría, F., Blaquier, J. A., Miranda, P. V. and Tezón, J. G. (2003), Effect of Antisperm Antibodies Present in Human Follicular Fluid upon the Acrosome Reaction and Sperm–Zona pellucida Interaction. American Journal of Reproductive Immunology, 50: 209–219. doi: 10.1034/j.1600-0897.2003.00082.x
- Issue published online: 11 AUG 2003
- Article first published online: 11 AUG 2003
- Submitted August 29, 2002; revised January 13, 2002; accepted January 15, 2003.
- Acrosome reaction;
- antisperm antibodies;
- follicular fluid;
- zona pellucida
Problem: To determine the ability of IgGs isolated from follicular fluids (hFFIgGs) to induce the acrosome reaction (AR) in human spermatozoa and to inhibit sperm–zona pellucida (ZP) interaction.
Method of study: Incubation of capacitated spermatozoa with hFFIgGs (n = 40) and assessment of their effect on the AR or hemizona (HZ) assay in a condition that allows sperm–ZP interaction, avoiding acrosomal exocytosis.
Results: hFFIgGs from different women varied in their ability of inducing the AR. Those hFFIgGs with the highest AR-inducing capacity evoked the exocytotic response in most of the different sperm donors tested [high Induction Frequency (IF)]. Some of these antibodies were also able of inhibiting sperm binding to ZP [low HZ Index (HZI)]. A significant correlation was found between the IF and the HZI for each hFFIgG.
Conclusions: Human follicular fluid contains antibodies capable of inducing the AR and inhibiting sperm–ZP binding, suggesting that they could be directed towards ZP receptors. hFFIgGs would constitute a tool for the identification of sperm entities involved in fertilization.