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Predominant Intracellular Expression of CXCR4 and CCR5 in Purified Primary Trophoblast Cells from First Trimester and Term Human Placentae

Authors

  • Juan Maldonado-Estrada,

    1. INSERM U 131, Equipe Cytokines et Relation Materno-Foetale, Hôpital Béclère, Clamart, France;
    2. Grupo de Reproducción Animal, Escuela de Medicina Veterinaria, Facultad de Ciencias Agrarias, Ciudadela de Robledo, Universidad de Antioquia, Medellín, Colombia;
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  • Elisabeth Menu,

    1. INSERM U 131, Equipe Cytokines et Relation Materno-Foetale, Hôpital Béclère, Clamart, France;
    2. Unité de Biologie des Rétrovirus, Institut Pasteur, Paris, France;
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  • Pierre Roques,

    1. Service of Neurovirology (SNV), Commissariat a l'Energie Atomique (CEA), Division Léclerc, Fontenay-aux-Roses, France;
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  • Bruno Vaslin,

    1. Service of Neurovirology (SNV), Commissariat a l'Energie Atomique (CEA), Division Léclerc, Fontenay-aux-Roses, France;
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  • Alice Dautry-Varsat,

    1. Unité de Biologie des Interactions Cellulaires, Institut Pasteur, Paris, France
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  • Françoise Barré-Sinoussi,

    1. Unité de Biologie des Rétrovirus, Institut Pasteur, Paris, France;
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  • Gérard Chaouat

    1. INSERM U 131, Equipe Cytokines et Relation Materno-Foetale, Hôpital Béclère, Clamart, France;
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Address reprint requests to Juan G. Maldonado-Estrada, Grupo de Reproducción Animal, Escuela de Medicina Veterinaria, Facultad de Ciencias Agrarias, Ciudadela de Robledo, Universidad de Antioquia, Medellín, Colombia.
E-mail: juanguimal@epm.net.co

Abstract

Problem: The aim of the present study was to define the expression of CXCR4 and CCR5 on non-cultured non-stimulated primary human trophoblast cells (TCs) immediately after their immunopurification.

Method of study: We have evaluated by flow cytometric analysis and immunofluorescence, highly purified primary TCs prepared from first trimester (8.2 ± 0.3 weeks, n = 15) and term (Caesarean section, n = 10) placentae for the cell surface and intracellular expression of CXCR4 and CCR5.

Results: There was a high level of individual variability for CXCR4 and CCR5 expression between trophoblast batches. In first trimester and term placentae TCs, we found a greater number of TCs preparations expressing intracellular CXCR4 than CCR5 (P < 0.05). Both receptors were predominantly localized in the intracellular compartment of TCs, whatever if isolated from first trimester or term placentae.

Conclusions: The functional consequences of the predominance of CXCR4 expression and of cellular addressing are briefly discussed.

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