Presented at the 8th Congress of the Alps Adria Society for Immunology of Reproduction, Weimar, Germany, September 2002.
Characterization of Spermatozoa Surface Antigens by Antisperm Antibodies and its Influence on Acrosomal Exocytosis
Article first published online: 17 OCT 2003
American Journal of Reproductive Immunology
Volume 50, Issue 5, pages 411–419, November 2003
How to Cite
Bohring, C. and Krause, W. (2003), Characterization of Spermatozoa Surface Antigens by Antisperm Antibodies and its Influence on Acrosomal Exocytosis. American Journal of Reproductive Immunology, 50: 411–419. doi: 10.1034/j.1600-0897.2003.00103.x
- Issue published online: 17 OCT 2003
- Article first published online: 17 OCT 2003
- Submitted March 26, 2002; revised September 25, 2002; accepted March 27, 2003.
- Antisperm antibodies;
- heat shock protein;
- isomerase ER-60
Problem: Antisperm antibodies (ASA) are the main cause of immunological infertility, they impair sperm functions by binding to the sperm membrane. The aim of this study was to characterize highly enriched sperm membrane proteins by 2-D-electrophoresis and to identify membrane antigens binding ASA and to evaluate the influence of ASA on the acrosome reaction (AR).
Method of study: Sperm membrane proteins were separated by 2-D-electrophoresis and antigens were identified by immunoblotting with ASA from seminal plasma samples of infertile men. The influence of ASA on the AR were observed and determined by means of flowcytometry.
Results: A total of 18 antigens were identified by using ASA from seminal plasma. Six of the recognized proteins were analyzed by means of mass spectrometry and peptide matching: HSP70 and HSP70-2, disulfide-isomerase-ER60, caspase-3 and two subunits of the proteasome (component-C2 and zeta-chain). ASA from seminal plasma are able to enhance the AR in donor-spermatozoa.
Conclusion: The biochemical identification of these proteins will be helpful to understand the mechanism by which ASA impair sperm function and the fertilization process. Spermatozoa, in which the AR was prematurely induced by ASA, will not be able to fertilize anymore.