Inhibitory effect of salvinorin A, from Salvia divinorum, on ileitis-induced hypermotility: cross-talk between κ-opioid and cannabinoid CB1 receptors
Article first published online: 29 JAN 2009
2008 British Pharmacological Society
British Journal of Pharmacology
Volume 155, Issue 5, pages 681–689, November 2008
How to Cite
Capasso, R., Borrelli, F., Cascio, M. G., Aviello, G., Huben, K., Zjawiony, J. K., Marini, P., Romano, B., Di Marzo, V., Capasso, F. and Izzo, A. A. (2008), Inhibitory effect of salvinorin A, from Salvia divinorum, on ileitis-induced hypermotility: cross-talk between κ-opioid and cannabinoid CB1 receptors. British Journal of Pharmacology, 155: 681–689. doi: 10.1038/bjp.2008.294
- Issue published online: 29 JAN 2009
- Article first published online: 29 JAN 2009
- (Received May 29, 2008, Accepted June 18, 2008)
- cannabinoid receptors;
- fatty acid amide hydrolase;
- endocannabinoid transport;
- inflammatory bowel disease;
- intestinal motility;
- κ-opioid receptors (KORs);
- myenteric plexus;
- Salvia divinorum;
- salvinorin A
Background and purpose:
Salvinorin A, the active component of the hallucinogenic herb Salvia divinorum, inhibits intestinal motility through activation of κ-opioid receptors (KORs). However, this compound may have target(s) other than the KORs in the inflamed gut. Because intestinal inflammation upregulates cannabinoid receptors and endogenous cannabinoids, in the present study we investigated the possible involvement of the endogenous cannabinoid system in salvinorin A-induced delay in motility in the inflamed gut.
Motility in vivo was measured by evaluating the distribution of a fluorescent marker along the small intestine; intestinal inflammation was induced by the irritant croton oil; direct or indirect activity at cannabinoid receptors was evaluated by means of binding, enzymic and cellular uptake assays.
Salvinorin A as well as the KOR agonist U-50488 reduced motility in croton oil treated mice. The inhibitory effect of both salvinorin A and U-50488 was counteracted by the KOR antagonist nor-binaltorphimine and by the cannabinoid CB1 receptor antagonist rimonabant. Rimonabant, however, did not counteract the inhibitory effect of salvinorin A on motility in control mice. Binding experiments showed very weak affinity of salvinorin A for cannabinoid CB1 and CB2 and no inhibitory effect on 2-arachidonoylglycerol and anandamide hydrolysis and cellular uptake.
Conclusions and implications:
The inhibitory effect of salvinorin A on motility reveals a functional interaction between cannabinoid CB1 receptors and KORs in the inflamed—but not in the normal—gut in vivo.
British Journal of Pharmacology (2008) 155, 681–689; doi:10.1038/bjp.2008.294; published online 14 July 2008