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Localisation of DivIVA by targeting to negatively curved membranes
Article first published online: 28 MAY 2009
Copyright © 2009 European Molecular Biology Organization
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission.
The EMBO Journal
Volume 28, Issue 15, pages 2272–2282, August 5, 2009
How to Cite
Lenarcic, R., Halbedel, S., Visser, L., Shaw, M., Wu, L. J., Errington, J., Marenduzzo, D. and Hamoen, L. W. (2009), Localisation of DivIVA by targeting to negatively curved membranes. The EMBO Journal, 28: 2272–2282. doi: 10.1038/emboj.2009.129
- Issue published online: 5 AUG 2009
- Article first published online: 28 MAY 2009
- Manuscript Accepted: 15 APR 2009
- Manuscript Received: 28 NOV 2008
- Bacillus subtilis;
- cell division;
- membrane curvature;
DivIVA is a conserved protein in Gram-positive bacteria and involved in various processes related to cell growth, cell division and spore formation. DivIVA is specifically targeted to cell division sites and cell poles. In Bacillus subtilis, DivIVA helps to localise other proteins, such as the conserved cell division inhibitor proteins, MinC/MinD, and the chromosome segregation protein, RacA. Little is known about the mechanism that localises DivIVA. Here we show that DivIVA binds to liposomes, and that the N terminus harbours the membrane targeting sequence. The purified protein can stimulate binding of RacA to membranes. In mutants with aberrant cell shapes, DivIVA accumulates where the cell membrane is most strongly curved. On the basis of electron microscopic studies and other data, we propose that this is due to molecular bridging of the curvature by DivIVA multimers. This model may explain why DivIVA localises at cell division sites. A Monte-Carlo simulation study showed that molecular bridging can be a general mechanism for binding of proteins to negatively curved membranes.