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msb201011-sup-0001.xlsapplication/xls465K

Supplementary Table 1

Log2 ratios of transcript fold changes between the GCR2 mutant and the wild-type. An average of three biological replicas is reported. (see text file)

msb201011-sup-0002.xlsapplication/xls18K

Supplementary Table 3

Log2 ratios of enzyme fold changes between the GCR2 mutant and the wild-type. An average of two biological replicas is reported. (see text file)

msb201011-sup-0003.xlsapplication/xls34K

Supplementary Table 4A

Metabolite concentration. Determined in exponentially grown wild-type FY4 and GCR2 mutant batch cultures on A) glucose minimal medium and ethanol minimal medium. The cdw to OD correlation was determined for wild-type FY4 and applied also to the GCR2 mutant. B) Metabolite fold changes of four strains with Tet-controlled promotor (Tet-controlled enzymes: Pgi1p, Tpi1p, Eno2p, Cdc19p) determined in glucose minimal medium with amino acids and varying concentrations of doxycycline. Data are normalized to the metabolite concentration determined without the addition doxycycline. (see text files)

msb201011-sup-0004.xlsapplication/xls24K

Supplementary Table 4B

Metabolite concentration. Determined in exponentially grown wild-type FY4 and GCR2 mutant batch cultures on A) glucose minimal medium and ethanol minimal medium. The cdw to OD correlation was determined for wild-type FY4 and applied also to the GCR2 mutant. B) Metabolite fold changes of four strains with Tet-controlled promotor (Tet-controlled enzymes: Pgi1p, Tpi1p, Eno2p, Cdc19p) determined in glucose minimal medium with amino acids and varying concentrations of doxycycline. Data are normalized to the metabolite concentration determined without the addition doxycycline. (see text files)

msb201011-sup-0005.xlsapplication/xls101K

Supplementary Table 5

SRM assays used in this study for measuring enzyme abundance alterations. Q1m/z, mass-to-charge ratio for the peptide ion used as Q1 value; Q3m/z mass-to-charge ratio for the fragmentation used as Q3 value; H/L, heavy/light version of the peptide; CE, collision energy. (see text file)

msb201011-sup-0006.xlsapplication/xls16K

Supplementary Table 6

SRM assays used in this study for measuring metabolite concentrations with liquid chromatography mass spectrometry. Q1m/z, mass-to-charge ratio for the metabolite ion used as Q1 value; Q3m/z mass-to-charge ratio for the fragmentation used as Q3 value. (see text file)

msb201011-sup-0007.pdfPDF document110K

Supplementary Information

Supplementary figures S1–2, Supplementary table legends S1–6, Supplementary table 2

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