Standard Article

Quantification of Metabolic Fluxes

  1. Andreas Karoly Gombert1,
  2. Jens Nielsen2

Published Online: 28 MAY 2003

DOI: 10.1038/npg.els.0000622

eLS

eLS

How to Cite

Gombert, A. K. and Nielsen, J. 2003. Quantification of Metabolic Fluxes. eLS. .

Author Information

  1. 1

    University of São Paulo, São Paulo, Brazil

  2. 2

    Technical University of Denmark, Lyngby, Denmark

Publication History

  1. Published Online: 28 MAY 2003

Abstract

The phenotype of an organism grown under defined environmental conditions may be characterized by the fluxes carried out at each individual reaction in the organism's metabolic network. Metabolic fluxes are nonmeasurable quantities, but they can be quantified using a combination of specific measurements and mathematical modelling.

Keywords:

  • metabolic flux analysis;
  • mathematical modelling of metabolism;
  • labelling experiments;
  • metabolic engineering