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Substrate Binding to Enzymes

  1. Vladimir K Pliška

Published Online: 25 APR 2001

DOI: 10.1038/npg.els.0000862



How to Cite

Pliška, V. K. 2001. Substrate Binding to Enzymes. eLS. .

Author Information

  1. Swiss Federal Institute of Technology (ETH), Zürich, Switzerland

Publication History

  1. Published Online: 25 APR 2001


Binding of a substrate to an enzyme is the first step of the interaction between substrate (ligand) and enzyme molecules that leads to the formation of an intermediate substrate–enzyme complex. The formation process is controlled mainly by diffusion and requires a structural complementarity between the enzyme and ligand.


  • substrate–enzyme complex;
  • rate constants;
  • dissociation constant;
  • allosteric interactions;
  • steady state;
  • Michaelis constant;
  • Hill equation;
  • Adair equation;
  • binding isotherm;
  • weak bonds;
  • hydrophobic interactions