Standard Article

Two-hybrid and Related Systems

  1. Stanley Fields

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0000981



How to Cite

Fields, S. 2001. Two-hybrid and Related Systems. eLS. .

Author Information

  1. University of Washington, Seattle, Washington, USA

Publication History

  1. Published Online: 19 APR 2001

This is not the most recent version of the article. View current version (15 MAY 2017)


The two-hybrid system is a yeast-based genetic method to detect and analyse protein–protein interactions. Related systems have been developed to detect DNA–protein, RNA–protein and small molecule–protein interactions.


  • protein–protein interaction;
  • DNA–protein interaction;
  • RNA–protein interaction;
  • yeast;
  • transcription factor