Phage Display Technologies
Published Online: 19 APR 2001
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Stricker, N. and Li, M. 2001. Phage Display Technologies. eLS. .
- Published Online: 19 APR 2001
Phage display is a method that uses bacterial virus (phage) as a vehicle to express diverse protein or peptide sequences as part of the phage coat protein by cloning deoxyribonucleic acid (DNA) fragments in frame with phage coat protein genes. Upon viral infection, the expressed peptides are displayed on the surface of the viral particle. Depending on the DNA fragments cloned, phage display allows for expression of either short peptides or large proteins, such as immunoglobulin, on the viral surface.