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Phage Display Technologies

  1. Nicole Stricker,
  2. Min Li

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0000982

eLS

eLS

How to Cite

Stricker, N. and Li, M. 2001. Phage Display Technologies. eLS. .

Author Information

  1. Johns Hopkins University, Baltimore, Maryland, USA

Publication History

  1. Published Online: 19 APR 2001

Abstract

Phage display is a method that uses bacterial virus (phage) as a vehicle to express diverse protein or peptide sequences as part of the phage coat protein by cloning deoxyribonucleic acid (DNA) fragments in frame with phage coat protein genes. Upon viral infection, the expressed peptides are displayed on the surface of the viral particle. Depending on the DNA fragments cloned, phage display allows for expression of either short peptides or large proteins, such as immunoglobulin, on the viral surface.

Keywords:

  • phage;
  • protein;
  • library;
  • antibody;
  • plasmid