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Immunoprecipitation Techniques

  1. Karl-Heinz Scheidtmann

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0001180

eLS

eLS

How to Cite

Scheidtmann, K.-H. 2001. Immunoprecipitation Techniques. eLS. .

Author Information

  1. University of Bonn, Germany

Publication History

  1. Published Online: 19 APR 2001

Abstract

Immunoprecipitations are based on two types of interaction: between antigen and antibody, and between antibody and an immunoadsorbent. The antigen–antibody complex will be bound to an insoluble matrix that can be pelleted and removed from the bulk protein. Immunoprecipitation allows rapid and efficient purification of proteins in a native and biochemically active form.

Keywords:

  • antibody;
  • antigen;
  • epitope;
  • immunoprecipitation;
  • protein A