Chromosome Preparation and Banding
Published Online: 19 APR 2001
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Moore, C. M. and Best, R. G. 2001. Chromosome Preparation and Banding. eLS. .
- Published Online: 19 APR 2001
Reliable techniques have been developed to produce large numbers of mitotic cells and to collect them at metaphase in order to visualize individual chromosomes. After cell culture and spreading of the metaphases onto slides, the chromosomes are stained to produce unique banding patterns or to reveal specialized structures. Molecular techniques have been developed to identify submicroscopic rearrangements and to compare karyotypes of different species.
- chromosome banding;
- chromosome spreading;
- fluorescence in situ hybridization;
- high resolution banding;
- metaphase spreads;
- sister chromatid exchange