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Chromosome Preparation and Banding

  1. Charleen M Moore1,
  2. Robert G Best2

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0001444



How to Cite

Moore, C. M. and Best, R. G. 2001. Chromosome Preparation and Banding. eLS. .

Author Information

  1. 1

    University of Texas Health Science Center at San Antonio, Texas, USA

  2. 2

    University of South Carolina School of Medicine, Columbia, South Carolina, USA

Publication History

  1. Published Online: 19 APR 2001


Reliable techniques have been developed to produce large numbers of mitotic cells and to collect them at metaphase in order to visualize individual chromosomes. After cell culture and spreading of the metaphases onto slides, the chromosomes are stained to produce unique banding patterns or to reveal specialized structures. Molecular techniques have been developed to identify submicroscopic rearrangements and to compare karyotypes of different species.


  • chromosome banding;
  • chromosome spreading;
  • fluorescence in situ hybridization;
  • high resolution banding;
  • metaphase spreads;
  • sister chromatid exchange