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Chromosome Preparation and Banding

  1. Charleen M Moore1,
  2. Robert G Best2

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0001444

eLS

eLS

How to Cite

Moore, C. M. and Best, R. G. 2001. Chromosome Preparation and Banding. eLS. .

Author Information

  1. 1

    University of Texas Health Science Center at San Antonio, Texas, USA

  2. 2

    University of South Carolina School of Medicine, Columbia, South Carolina, USA

Publication History

  1. Published Online: 19 APR 2001
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Figure 1. G-Banding (a) Normal human male metaphase spread showing 46 human chromosomes. G-Bands were produced by treatment with trypsin followed by staining with Giemsa. (b) The same metaphase arranged in standard karyotype format.

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Figure 2. Molecular cytogenetic probes. (a) Normal human metaphase spread showing hybridization of the centromeric region of chromosomes 7 (green) and 8 (red) using α-satellite probes. Chromosomes counterstained in blue using DAPI. (b) Human male metaphase spread with deletion of the elastin (ELN) locus at the Williams syndrome critical region near the centromere on one homologue of chromosome 7. Cosmid probe for the ELN locus and a control probe are visible on the normal homologue (right); however, only the control probe can be seen on the deleted homologue (left). (c) Normal human metaphase spread with whole-chromosome paint probe for chromosome pair number 15 in red. (d) Cross-species colour banding (RX-FISH®) on normal human male chromosomes arranged in standard karyotype format.