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Agrobacterium tumefaciens-mediated Transformation of Plant Cells

  1. Andrew Binns,
  2. Angela Campbell

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0001492

eLS

eLS

How to Cite

Binns, A. and Campbell, A. 2001. Agrobacterium tumefaciens-mediated Transformation of Plant Cells. eLS. .

Author Information

  1. University of Pennsylvania, Philadelphia, Pennsylvania, USA

Publication History

  1. Published Online: 19 APR 2001

Abstract

Agrobacterium tumefaciens is a Gram-negative soil bacterium that causes plant tumours by transferring a portion of DNA from a resident ‘tumour inducing’ (Ti) plasmid into plant cells where it is integrated into a plant chromosome and expressed. This bacteria's capacity for DNA transfer is the basis of most current plant genetic engineering and make it a model system for the study of pathogenic bacteria that transfer virulence factors to any eukaryotic cell.

Keywords:

  • agrobacterium tumefaciens;
  • ti plasmid;
  • DNA transfer;
  • plant genetic engineering;
  • crown gall tumours