Standard Article

hnRNP: Methods for Preparation

  1. Jia-huai Tan1,
  2. Wallace M LeStourgeon2

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0002603

eLS

eLS

How to Cite

Tan, J.-h. and LeStourgeon, W. M. 2001. hnRNP: Methods for Preparation. eLS. .

Author Information

  1. 1

    Vanderbilt University, Nashville, Tennessee, USA

  2. 2

    Vanderbilt University, Nashville, Tennessee, USA

Publication History

  1. Published Online: 19 APR 2001

Abstract

During transcription in higher eukaryotes, the elongating pre-messenger RNA is bound by a unique set of abundant nuclear proteins to form a repeating array of 20–22-nm particles termed 30–40S heterogeneous nuclear ribonucleoprotein (hnRNP) particles. A variety of methods exist for the isolation and purification of hnRNP complexes and for the in vitro assembly of hnRNP assembly intermediates.

Keywords:

  • ribonucleoprotein;
  • hnRNA;
  • hnRNP;
  • pre-mRNA;
  • RNA-binding protein