Standard Article

snRNPs: Methods for Purification

  1. Berthold Kastner

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0002605

eLS

eLS

How to Cite

Kastner, B. 2001. snRNPs: Methods for Purification. eLS. .

Author Information

  1. Phillipps University of Marburg, Marburg, Germany

Publication History

  1. Published Online: 19 APR 2001

Abstract

Small nuclear ribonucleoprotein particles (snRNPs) are essential cofactors for the nuclear splicing of pre-mRNA introns. The method best suited to the purification of snRNPs is affinity chromatography combined with competitive elution.

Keywords:

  • snRNP;
  • pre-mRNA splicing;
  • chromatography;
  • RNA–protein interaction