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DNA: Methods for Preparation

  1. Michael Mingfu Ling

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0002606

eLS

eLS

How to Cite

Ling, M. M. 2001. DNA: Methods for Preparation. eLS. .

Author Information

  1. Spectral Diagnostics Inc., Toronto, Canada

Publication History

  1. Published Online: 19 APR 2001

Abstract

The isolation of DNA is required for genetic analysis and genetic engineering. There are primarily two types of method for isolating genomic DNA: (i) proteinase K digestion in the presence of SDS and EDTA at temperatures between 50 and 60°C; (ii) the use of strong chaotropic reagents such as guanidinium chloride. The trend is towards methods that are amenable to high-throughput screening and automation.

Keywords:

  • DNA;
  • genomic;
  • isolation;
  • genetic analysis;
  • genetic engineering