DNA: Methods for Preparation
Published Online: 19 APR 2001
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Ling, M. M. 2001. DNA: Methods for Preparation. eLS. .
- Published Online: 19 APR 2001
The isolation of DNA is required for genetic analysis and genetic engineering. There are primarily two types of method for isolating genomic DNA: (i) proteinase K digestion in the presence of SDS and EDTA at temperatures between 50 and 60°C; (ii) the use of strong chaotropic reagents such as guanidinium chloride. The trend is towards methods that are amenable to high-throughput screening and automation.
- genetic analysis;
- genetic engineering