Standard Article

Ion Exchange Chromatography

  1. David Sheehan,
  2. Siobhan O'Sullivan

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0002677

eLS

eLS

How to Cite

Sheehan, D. and O'Sullivan, S. 2001. Ion Exchange Chromatography. eLS. .

Author Information

  1. University College Cork, Mardyke, Cork, Ireland

Publication History

  1. Published Online: 19 APR 2001

Abstract

Ion exchange chromatography is a method for separating complex mixtures on the basis of differing tendencies of individual charged molecules (ions) to exchange with like-charged species bound by electrostatic attraction to groups immobilized on a stationary phase. It is especially suitable for purification of proteins as their net charge may be altered by varying pH.

Keywords:

  • bioseparation;
  • chromatography;
  • displacers;
  • ion exchange;
  • perfusion;
  • protein;
  • membrane