Ion Exchange Chromatography
Published Online: 19 APR 2001
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Sheehan, D. and O'Sullivan, S. 2001. Ion Exchange Chromatography. eLS. .
- Published Online: 19 APR 2001
Ion exchange chromatography is a method for separating complex mixtures on the basis of differing tendencies of individual charged molecules (ions) to exchange with like-charged species bound by electrostatic attraction to groups immobilized on a stationary phase. It is especially suitable for purification of proteins as their net charge may be altered by varying pH.
- ion exchange;