Standard Article


  1. Douglas D Frey,
  2. Chittoor R Narahari,
  3. Ronald C Bates

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0002681



How to Cite

Frey, D. D., Narahari, C. R. and Bates, R. C. 2001. Chromatofocusing. eLS. .

Author Information

  1. University of Maryland Baltimore County, Maryland, USA

Publication History

  1. Published Online: 19 APR 2001

This is not the most recent version of the article. View current version (14 MAY 2015)


Chromatofocusing is a form of gradient elution chromatography performed using an ion-exchange column packing and an internally generated pH gradient that travels through the column as a retained pH front.


  • chromatofocusing;
  • chromatography;
  • preparative chromatography;
  • pH gradient;
  • protein purification;
  • polyampholyte buffer