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Gel Electrophoresis of Proteins: High-resolution Two-dimensional

  1. Julio E Celis,
  2. Pavel S Gromov

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0002684

eLS

eLS

How to Cite

Celis, J. E. and Gromov, P. S. 2001. Gel Electrophoresis of Proteins: High-resolution Two-dimensional. eLS. .

Author Information

  1. Danish Centre for Human Genome Research, Aarhus, Denmark

Publication History

  1. Published Online: 19 APR 2001

Abstract

The technique of high-resolution two-dimensional gel electrophoresis separates proteins according to both isoelectric point and molecular weight, giving it unsurpassed resolving power.

Keywords:

  • 2D PAGE;
  • isoelectric focusing (IEF);
  • nonequilibrium pH gradient;
  • immobilized pH gradient