Autoradiography and Fluorography
Published Online: 26 MAR 2003
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Gromov, P. S. and Celis, J. E. 2003. Autoradiography and Fluorography. eLS. .
- Published Online: 26 MAR 2003
Radioactive compounds are used to detect and monitor labelled cellular components by imaging on photographic emulsion in contact with the sample. In direct autoradiography, emissions from high-energy radioisotopes directly affect the emulsion. In indirect autoradiography, a fluorescent intensifying screen produces photons that affect the emulsion; and in fluorography the emulsion detects photons produced when radioactive emissions interact with an added scintillant and the techique can use low-energy emitters.
- radioactive isotopes;
- X-ray film;
- intensifying screen;