Standard Article

Autoradiography and Fluorography

  1. Pavel S Gromov,
  2. Julio E Celis

Published Online: 26 MAR 2003

DOI: 10.1038/npg.els.0002685

eLS

eLS

How to Cite

Gromov, P. S. and Celis, J. E. 2003. Autoradiography and Fluorography. eLS. .

Author Information

  1. University of Aarhus, Aarhus, Denmark

Publication History

  1. Published Online: 26 MAR 2003

Abstract

Radioactive compounds are used to detect and monitor labelled cellular components by imaging on photographic emulsion in contact with the sample. In direct autoradiography, emissions from high-energy radioisotopes directly affect the emulsion. In indirect autoradiography, a fluorescent intensifying screen produces photons that affect the emulsion; and in fluorography the emulsion detects photons produced when radioactive emissions interact with an added scintillant and the techique can use low-energy emitters.

Keywords:

  • radioactive isotopes;
  • X-ray film;
  • radioactivity;
  • intensifying screen;
  • phosphorimager