Protein Identification: Overlay Procedures
Published Online: 19 APR 2001
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Gromov, P. S. and Celis, J. E. 2001. Protein Identification: Overlay Procedures. eLS.
- Published Online: 19 APR 2001
The protein blot overlay assay is a powerful technique for identifying proteins transferred to an immobilizing matrix. It is based on the specific binding of labelled ligands with proteins of interest blotted onto the membrane, followed by detection of the complexes formed.
- protein–ligand interactions;
- blot overlay assay