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Protein Identification: Overlay Procedures

  1. Pavel S Gromov,
  2. Julio E Celis

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0002689



How to Cite

Gromov, P. S. and Celis, J. E. 2001. Protein Identification: Overlay Procedures. eLS. .

Author Information

  1. University of Aarhus, Aarhus, Denmark

Publication History

  1. Published Online: 19 APR 2001


The protein blot overlay assay is a powerful technique for identifying proteins transferred to an immobilizing matrix. It is based on the specific binding of labelled ligands with proteins of interest blotted onto the membrane, followed by detection of the complexes formed.


  • protein–ligand interactions;
  • blot overlay assay