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Transcription and Translation In Vitro

  1. Martin J Tymms

Published Online: 25 APR 2001

DOI: 10.1038/npg.els.0002691

eLS

eLS

How to Cite

Tymms, M. J. 2001. Transcription and Translation In Vitro. eLS. .

Author Information

  1. National Vision Research Institute, Melbourne, Australia

Publication History

  1. Published Online: 25 APR 2001

Abstract

In vitro transcription–translation provides the means to synthesize proteins rapidly from a DNA template sequence. Using polymerase chain reaction, DNA templates can be generated rapidly from any coding sequence and protein produced without the use of time-consuming protein expression systems using living cells.

Keywords:

  • rabbit reticulocyte lysate;
  • T7 polymerase;
  • T7 promoter;
  • protein truncation test