Transcription and Translation In Vitro
Published Online: 25 APR 2001
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Tymms, M. J. 2001. Transcription and Translation In Vitro. eLS.
- Published Online: 25 APR 2001
In vitro transcription–translation provides the means to synthesize proteins rapidly from a DNA template sequence. Using polymerase chain reaction, DNA templates can be generated rapidly from any coding sequence and protein produced without the use of time-consuming protein expression systems using living cells.
- rabbit reticulocyte lysate;
- T7 polymerase;
- T7 promoter;
- protein truncation test