Standard Article

Nuclear Pores: Methods for Preparation

  1. Cécile Fradin

Published Online: 15 JUL 2003

DOI: 10.1038/npg.els.0002706

eLS

eLS

How to Cite

Fradin, C. 2003. Nuclear Pores: Methods for Preparation. eLS. .

Author Information

  1. McMaster University, Hamilton, Ontario, Canada

Publication History

  1. Published Online: 15 JUL 2003

Abstract

Nuclear pore complexes are large protein assemblies that span the nuclear envelope and are involved in the regulation of molecular transport between the nucleus and the cytoplasm. There are three different methods that allow preparation of intact functional nuclear pore complexes: cell permeabilization, preparation of isolated nuclei, and cell-free nuclear reconstitution.

Keywords:

  • nuclear pore complex;
  • nuclear envelope;
  • nucleus;
  • nuclear transport;
  • intracellular trafficking