Nuclear Pores: Methods for Preparation
Published Online: 15 JUL 2003
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Fradin, C. 2003. Nuclear Pores: Methods for Preparation. eLS. .
- Published Online: 15 JUL 2003
Nuclear pore complexes are large protein assemblies that span the nuclear envelope and are involved in the regulation of molecular transport between the nucleus and the cytoplasm. There are three different methods that allow preparation of intact functional nuclear pore complexes: cell permeabilization, preparation of isolated nuclei, and cell-free nuclear reconstitution.
- nuclear pore complex;
- nuclear envelope;
- nuclear transport;
- intracellular trafficking