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Two-photon Fluorescence Light Microscopy

  1. Peter TC So

Published Online: 30 MAY 2001

DOI: 10.1038/npg.els.0002991

eLS

eLS

How to Cite

So, P. T. 2001. Two-photon Fluorescence Light Microscopy. eLS. .

Author Information

  1. Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

Publication History

  1. Published Online: 30 MAY 2001

This is not the most recent version of the article. View current version (15 JUN 2012)

Abstract

Two-photon fluorescence microscopy allows three-dimensional imaging of biological specimens in vivo. Compared with confocal microscopy, it offers the advantages of deeper tissue penetration and less photodamage but has the disadvantage of slightly lower resolution.

Keywords:

  • two-photon;
  • microscopy;
  • tissue optics;
  • fluorescence