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Two-photon Fluorescence Light Microscopy

  1. Peter TC So

Published Online: 30 MAY 2001

DOI: 10.1038/npg.els.0002991



How to Cite

So, P. T. 2001. Two-photon Fluorescence Light Microscopy. eLS. .

Author Information

  1. Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

Publication History

  1. Published Online: 30 MAY 2001

This is not the most recent version of the article. View current version (23 DEC 2015)


Two-photon fluorescence microscopy allows three-dimensional imaging of biological specimens in vivo. Compared with confocal microscopy, it offers the advantages of deeper tissue penetration and less photodamage but has the disadvantage of slightly lower resolution.


  • two-photon;
  • microscopy;
  • tissue optics;
  • fluorescence