Standard Article

Deconvolution Fluorescence Light Microscopy

  1. Jason R Swedlow

Published Online: 22 MAR 2002

DOI: 10.1038/npg.els.0002992

eLS

eLS

How to Cite

Swedlow, J. R. 2002. Deconvolution Fluorescence Light Microscopy. eLS. .

Author Information

  1. University of Dundee, Dundee, Scotland, UK

Publication History

  1. Published Online: 22 MAR 2002

Abstract

Deconvolution microscopy is a computational technique that deblurs fluorescence images using knowledge of the optical path of the microscope.

Keywords:

  • deconvolution;
  • light microscopy;
  • point spread function;
  • three-dimensional imaging;
  • image restoration