Amino Acid Substitutions: Effects on Protein Stability
Published Online: 19 APR 2001
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Weng, Z. and DeLisi, C. 2001. Amino Acid Substitutions: Effects on Protein Stability. eLS.
- Published Online: 19 APR 2001
The ability of a protein to function in its biochemical role(s) is determined by its geometry, which in turn is determined by the amino acid sequence and the environment. Amino acid mutations affect the thermodynamics of folding and their effects can be investigated experimentally by such techniques as site-specific mutation, random point mutations and shuffling.
- protein stability;
- folding free energy;
- electrostatic interactions;
- site-specific mutagenesis;
- random mutations