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Amino Acid Substitutions: Effects on Protein Stability

  1. Zhiping Weng,
  2. Charles DeLisi

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0003006

eLS

eLS

How to Cite

Weng, Z. and DeLisi, C. 2001. Amino Acid Substitutions: Effects on Protein Stability. eLS. .

Author Information

  1. Boston University, Boston, Massachusetts, USA

Publication History

  1. Published Online: 19 APR 2001

Abstract

The ability of a protein to function in its biochemical role(s) is determined by its geometry, which in turn is determined by the amino acid sequence and the environment. Amino acid mutations affect the thermodynamics of folding and their effects can be investigated experimentally by such techniques as site-specific mutation, random point mutations and shuffling.

Keywords:

  • protein stability;
  • folding free energy;
  • solvation;
  • electrostatic interactions;
  • site-specific mutagenesis;
  • random mutations