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Mass Spectrometry: Analysis of Two-dimensional Protein Gels

  1. Hanjo Lim1,
  2. John R Yates III2

Published Online: 24 OCT 2002

DOI: 10.1038/npg.els.0003110

eLS

eLS

How to Cite

Lim, H. and Yates, J. R. 2002. Mass Spectrometry: Analysis of Two-dimensional Protein Gels. eLS. .

Author Information

  1. 1

    Aventis Pharmaceuticals Inc., Bridgewater, New Jersey, USA

  2. 2

    The Scripps Research Institute, La Jolla, California, USA

Publication History

  1. Published Online: 24 OCT 2002

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Abstract

Matrix-assisted laser desorption/ionization mass spectrometry and electrospray tandem mass spectrometry, coupled with liquid chromatography, enable high-throughput protein identification and further characterization, such as post-translational modification, of proteins, after initial separation by two-dimensional gel electrophoresis.

Keywords:

  • proteomics;
  • two-dimensional gel electrophoresis;
  • mass spectrometry;
  • posttranslational modification