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Proteins: Affinity Tags

  1. Shaorong Chong

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0003141

eLS

eLS

How to Cite

Chong, S. 2001. Proteins: Affinity Tags. eLS. .

Author Information

  1. New England Biolabs Inc., Beverly, Massachusetts, USA

Publication History

  1. Published Online: 19 APR 2001

Abstract

Specific interaction between a protein (or a protein domain) and a ligand allows the protein (or domain) to be linked to a recombinant protein as a fusion partner (affinity tag). This enables the recombinant protein to be purified by affinity chromatography even if it does not naturally have a specific ligand suitable for this process.

Keywords:

  • recombinant protein;
  • purification;
  • affinity tag