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Biological Macromolecules: UV-visible Spectrophotometry

  1. Franz-Xaver Schmid

Published Online: 19 APR 2001

DOI: 10.1038/npg.els.0003142



How to Cite

Schmid, F.-X. 2001. Biological Macromolecules: UV-visible Spectrophotometry. eLS. .

Author Information

  1. University of Bayreuth, Germany

Publication History

  1. Published Online: 19 APR 2001


Biological macromolecules such as proteins and nucleic acids absorb light in the UV-visible region of the spectrum. Absorbance measurements are used for measuring concentrations, for the detection of conformational changes and of ligand binding, and for following enzyme reactions.


  • absorbance;
  • proteins;
  • nucleic acids;
  • enzymes;
  • denaturation