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Nucleic Acids: Subtractive Hybridization

  1. Richard J Byers,
  2. Judith A Hoyland,
  3. Anthony J Freemont

Published Online: 3 MAY 2005

DOI: 10.1038/npg.els.0003149

eLS

eLS

How to Cite

Byers, R. J., Hoyland, J. A. and Freemont, A. J. 2005. Nucleic Acids: Subtractive Hybridization. eLS. .

Author Information

  1. University of Manchester, Manchester, UK

Publication History

  1. Published Online: 3 MAY 2005

Abstract

Cells can be characterized phenotypically by demonstrating expressed proteins or messenger RNA. ‘Subtracting’ the gene expression profile of one cell from that of a different cell eliminates the signal from common shared ‘housekeeping’ genes and allows the functional gene expression profile of one cell to be defined. Subtractive hybridization is one of the major methods for achieving this.

Keywords:

  • subtraction;
  • hybridization;
  • phenotyping;
  • poly(A) PCR;
  • mRNA