Nucleic Acids: Subtractive Hybridization
Published Online: 3 MAY 2005
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Byers, R. J., Hoyland, J. A. and Freemont, A. J. 2005. Nucleic Acids: Subtractive Hybridization. eLS. .
- Published Online: 3 MAY 2005
Cells can be characterized phenotypically by demonstrating expressed proteins or messenger RNA. ‘Subtracting’ the gene expression profile of one cell from that of a different cell eliminates the signal from common shared ‘housekeeping’ genes and allows the functional gene expression profile of one cell to be defined. Subtractive hybridization is one of the major methods for achieving this.
- poly(A) PCR;