Published Online: 27 JAN 2006
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Hamill, O. P. 2006. Patch-clamp Technique. eLS. .
- Published Online: 27 JAN 2006
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The patch-clamp technique enables the measurement of single membrane ion channel currents that underlie a wide variety of electrical and ionic signalling mechanisms in living cells. Biophysically, it provides the unique opportunity to follow in real time the conformational transitions of single membrane channel proteins with microsecond resolution and can be used as a functional membrane protein assay that is far more sensitive than traditional methods.
- patch clamp;
- single channel currents