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Patch-clamp Technique

  1. Owen P Hamill

Published Online: 27 JAN 2006

DOI: 10.1038/npg.els.0003382



How to Cite

Hamill, O. P. 2006. Patch-clamp Technique. eLS. .

Author Information

  1. University of Texas Medical Branch, Galveston, Texas, USA

Publication History

  1. Published Online: 27 JAN 2006

This is not the most recent version of the article. View current version (15 APR 2014)


The patch-clamp technique enables the measurement of single membrane ion channel currents that underlie a wide variety of electrical and ionic signalling mechanisms in living cells. Biophysically, it provides the unique opportunity to follow in real time the conformational transitions of single membrane channel proteins with microsecond resolution and can be used as a functional membrane protein assay that is far more sensitive than traditional methods.


  • patch clamp;
  • single channel currents