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Electrophoresis and Blotting of DNA

  1. Sandeep Tamber,
  2. Robert EW Hancock

Published Online: 11 MAR 2004

DOI: 10.1038/npg.els.0003746

eLS

eLS

How to Cite

Tamber, S. and Hancock, R. E. 2004. Electrophoresis and Blotting of DNA. eLS. .

Author Information

  1. University of British Columbia, Vancouver, Canada

Publication History

  1. Published Online: 11 MAR 2004

This is not the most recent version of the article. View current version (8 DEC 2013)

Abstract

DNA electrophoresis and blotting are techniques commonly used to visualize DNA. Electrophoresis uses gels to separate DNA molecules on the basis of size; whereas blotting allows researchers to identify specific DNA fragments on the basis of their sequences.

Keywords:

  • DNA detection;
  • DNA analysis;
  • gel electrophoresis;
  • alkaline/Southern blotting;
  • hybridization