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Nick Translation

  1. K Woodward

Published Online: 22 JUL 2003

DOI: 10.1038/npg.els.0003748



How to Cite

Woodward, K. 2003. Nick Translation. eLS. .

Author Information

  1. Institute of Child Health, London, UK

Publication History

  1. Published Online: 22 JUL 2003


Nick translation is a rapid and easy method of labelling duplex DNA with radioactive or chemically modified nucleotides. The technique is based on the ability of DNase I to introduce random nicks in the target DNA and the simultaneous exonuclease and polymerase activity of Escherichia coli DNA polymerase I to replace existing nucleotides with labelled nucleotides (Kelly et al., 1970; Rigby et al., 1977).


  • DNA labelling;
  • DNase I;
  • nick translation