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Denaturing Gel Electrophoresis of DNA in Alkaline Agarose Gels

  1. Edwin M Southern

Published Online: 24 OCT 2002

DOI: 10.1038/npg.els.0003758

eLS

eLS

How to Cite

Southern, E. M. 2002. Denaturing Gel Electrophoresis of DNA in Alkaline Agarose Gels. eLS. .

Author Information

  1. Department of Biochemistry, University of Oxford, Oxford, UK

Publication History

  1. Published Online: 24 OCT 2002

Abstract

One of the simplest ways to separate DNA under denaturing conditions is to use an alkaline agarose gel. This method is not, however, suitable for RNA because it is rapidly hydrolysed in alkaline conditions. Similarly, DNA containing ribonucleotides will be nicked.

Keywords:

  • RNA/DNA;
  • denature;
  • alkaline agarose