Denaturing Gel Electrophoresis of DNA in Alkaline Agarose Gels
Published Online: 24 OCT 2002
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Southern, E. M. 2002. Denaturing Gel Electrophoresis of DNA in Alkaline Agarose Gels. eLS. .
- Published Online: 24 OCT 2002
One of the simplest ways to separate DNA under denaturing conditions is to use an alkaline agarose gel. This method is not, however, suitable for RNA because it is rapidly hydrolysed in alkaline conditions. Similarly, DNA containing ribonucleotides will be nicked.
- alkaline agarose