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Antibody Purification by Ammonium Sulfate Precipitation

  1. JE Liddell

Published Online: 22 JUL 2003

DOI: 10.1038/npg.els.0003761

eLS

eLS

How to Cite

Liddell, J. 2003. Antibody Purification by Ammonium Sulfate Precipitation. eLS. .

Author Information

  1. University of Cardiff, Cardiff, UK

Publication History

  1. Published Online: 22 JUL 2003

Abstract

Ammonium sulfate precipitation of an antiserum or ascitic fluid will provide a crude immunoglobulin fraction free of other serum proteins. Purity is sufficient for the immunoglobulin to be used for labelling procedures or coating to an assay plate.

Keywords:

  • antibody purification;
  • ammonium sulfate;
  • salt precipitation