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Enzyme-linked Immunosorbent Assay (ELISA) to Measure Pure Protein

  1. JE Liddell

Published Online: 28 MAY 2003

DOI: 10.1038/npg.els.0003764

eLS

eLS

How to Cite

Liddell, J. 2003. Enzyme-linked Immunosorbent Assay (ELISA) to Measure Pure Protein. eLS. .

Author Information

  1. School of Biosciences, University of Cardiff, Cardiff, UK

Publication History

  1. Published Online: 28 MAY 2003

Abstract

Enzyme-linked immunosorbent assay (ELISA) is used to measure the concentration of a soluble protein. The protein is coated to an assay plate and detected using a three-step detection process. A colour change is observed which is proportional to the concentration of protein bound to the plate.

Keywords:

  • ELISA;
  • immunoassay;
  • protein measurement