Enzyme-linked Immunosorbent Assay (ELISA) to Measure Pure Protein
Published Online: 28 MAY 2003
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Liddell, J. 2003. Enzyme-linked Immunosorbent Assay (ELISA) to Measure Pure Protein. eLS. .
- Published Online: 28 MAY 2003
Enzyme-linked immunosorbent assay (ELISA) is used to measure the concentration of a soluble protein. The protein is coated to an assay plate and detected using a three-step detection process. A colour change is observed which is proportional to the concentration of protein bound to the plate.
- protein measurement