Standard Article

Conjugation of Proteins with Horseradish Peroxidase (HRP)

  1. Tor Gjøen,
  2. Trond Berg

Published Online: 22 JUL 2003

DOI: 10.1038/npg.els.0003770

eLS

eLS

How to Cite

Gjøen, T. and Berg, T. 2003. Conjugation of Proteins with Horseradish Peroxidase (HRP). eLS. .

Author Information

  1. University of Oslo, Oslo, Norway

Publication History

  1. Published Online: 22 JUL 2003

Abstract

The intracellular pathway of endocytosed ligands can be followed by density shift methods. When the ligand is conjugated to an enzyme like horseradish peroxidase (HRP), the organelle can be made denser by the addition of a substrate for this enzyme, diaminobenzidine (DAB). When this substrate is metabolized in the presence of hydrogen peroxide (H2O2), an insoluble aggregate is formed and the organelle will increase in density.

Keywords:

  • endocytosis;
  • cell fractionation;
  • peroxidase;
  • density shift;
  • lysosome;
  • conjugation