Standard Article

Direct Labelling of Proteins with 125I

  1. Tor Gjøen1,
  2. Trond Berg2

Published Online: 22 JUL 2003

DOI: 10.1038/npg.els.0003771

eLS

eLS

How to Cite

Gjøen, T. and Berg, T. 2003. Direct Labelling of Proteins with 125I. eLS. .

Author Information

  1. 1

    University of Oslo, Oslo, Norway

  2. 2

    University of Oslo, Oslo, Norway

Publication History

  1. Published Online: 22 JUL 2003

Abstract

To quantify binding to receptors and endocytosis of protein ligands, radioactive labelling with 125I is still the most widely used technique. It combines high sensitivity with simple analysis and is the method of choice for most applications. Radioactive iodine can be directly coupled to tyrosine residues or via adducts that accumulate in the cells at their site of degradation.

Keywords:

  • endocytosis;
  • protein labelling;
  • receptor;
  • ligand;
  • iodination;
  • protein degradation