Direct Labelling of Proteins with 125I
Published Online: 22 JUL 2003
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Gjøen, T. and Berg, T. 2003. Direct Labelling of Proteins with 125I. eLS. .
- Published Online: 22 JUL 2003
To quantify binding to receptors and endocytosis of protein ligands, radioactive labelling with 125I is still the most widely used technique. It combines high sensitivity with simple analysis and is the method of choice for most applications. Radioactive iodine can be directly coupled to tyrosine residues or via adducts that accumulate in the cells at their site of degradation.
- protein labelling;
- protein degradation