Affinity Purification of Antibodies
Published Online: 28 MAY 2003
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Liddell, J. 2003. Affinity Purification of Antibodies. eLS. .
- Published Online: 28 MAY 2003
This method produces the purest sample of antibody, containing only antibodies that react with the desired antigen, free of all other immunoglobulins. It is based on affinity chromatography, in which antigen is bound to a solid phase to which specific antibody is bound and then eluted off.
- affinity chromatography;
- antibody purification;