Standard Article

Affinity Purification of Antibodies

  1. JE Liddell

Published Online: 28 MAY 2003

DOI: 10.1038/npg.els.0003774



How to Cite

Liddell, J. 2003. Affinity Purification of Antibodies. eLS. .

Author Information

  1. Cardiff School of Biosciences, Cardiff University, Cardiff, UK

Publication History

  1. Published Online: 28 MAY 2003


This method produces the purest sample of antibody, containing only antibodies that react with the desired antigen, free of all other immunoglobulins. It is based on affinity chromatography, in which antigen is bound to a solid phase to which specific antibody is bound and then eluted off.


  • affinity chromatography;
  • antibody purification;
  • cyanogen-bromide