Denaturing Gel Electrophoresis of RNA and DNA Using Urea–Polyacrylamide Gels
Published Online: 24 OCT 2002
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Southern, E. M. 2002. Denaturing Gel Electrophoresis of RNA and DNA Using Urea–Polyacrylamide Gels. eLS. .
- Published Online: 24 OCT 2002
At high concentrations, urea is able to denature both DNA and RNA and polyacrylamide gels containing urea at 7 mol L−1 can be used to separate nucleic acids under denaturing conditions.