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Denaturing Gel Electrophoresis of RNA and DNA Using Urea–Polyacrylamide Gels

  1. Edwin M Southern

Published Online: 24 OCT 2002

DOI: 10.1038/npg.els.0003778

eLS

eLS

How to Cite

Southern, E. M. 2002. Denaturing Gel Electrophoresis of RNA and DNA Using Urea–Polyacrylamide Gels. eLS. .

Author Information

  1. University of Oxford, Oxford, UK

Publication History

  1. Published Online: 24 OCT 2002

Abstract

At high concentrations, urea is able to denature both DNA and RNA and polyacrylamide gels containing urea at 7 mol L−1 can be used to separate nucleic acids under denaturing conditions.

Keywords:

  • RNA;
  • DNA;
  • electrophoresis;
  • urea–polyacrylamide