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Pulsed-field Gel Electrophoresis of DNA

  1. Edwin M Southern

Published Online: 26 MAR 2003

DOI: 10.1038/npg.els.0003782

eLS

eLS

How to Cite

Southern, E. M. 2003. Pulsed-field Gel Electrophoresis of DNA. eLS. .

Author Information

  1. University of Oxford, Oxford, UK

Publication History

  1. Published Online: 26 MAR 2003

Abstract

Conventional electrophoresis is not useful for DNA larger than 50 000 base pairs (bp). For large DNA molecules up to 7 million bp (7 Mb) it is necessary to use pulsed-field electrophoresis. This protocol describes how the procedure is carried out.

Keywords:

  • DNA;
  • separation;
  • PFGE;
  • FIGE