Site-directed Mutagenesis Using PCR
Published Online: 22 JUL 2003
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Jones, P. 2003. Site-directed Mutagenesis Using PCR. eLS. .
- Published Online: 22 JUL 2003
PCR-based site-directed mutagenesis is used to study gene expression, protein structure–function relationships, and vector modification. This technique uses a three-stage PCR with optimized parameters for each stage and the use of a ddNTP-blocked fragment to produce a final mutant product.
- gene expression;
- structure–function relationships;
- vector modification;
- three-stage PCR