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Site-directed Mutagenesis Using PCR

  1. P Jones

Published Online: 22 JUL 2003

DOI: 10.1038/npg.els.0003784

eLS

eLS

How to Cite

Jones, P. 2003. Site-directed Mutagenesis Using PCR. eLS. .

Author Information

  1. Zeneca Agrochemicals, Bracknell, UK

Publication History

  1. Published Online: 22 JUL 2003

Abstract

PCR-based site-directed mutagenesis is used to study gene expression, protein structure–function relationships, and vector modification. This technique uses a three-stage PCR with optimized parameters for each stage and the use of a ddNTP-blocked fragment to produce a final mutant product.

Keywords:

  • gene expression;
  • structure–function relationships;
  • vector modification;
  • three-stage PCR