Standard Article

Electroblotting of Gels

  1. Edwin M Southern

Published Online: 22 JUL 2003

DOI: 10.1038/npg.els.0003790

eLS

eLS

How to Cite

Southern, E. M. 2003. Electroblotting of Gels. eLS. .

Author Information

  1. University of Oxford, Oxford, UK

Publication History

  1. Published Online: 22 JUL 2003

Abstract

Quite often the gel electrophoretic migration pattern of individual components in a sample is determined not by analysing the gel but rather by analysing an image of the gel created by blotting the gel on to membrane. One of the simplest procedures is capillary blotting but this takes several hours and cannot be used with polyacrylamide gels. The transfer of nucleic acids can be speeded up by electroblotting.

Keywords:

  • RNA;
  • DNA;
  • blotting