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Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane

  1. J Beauchamp

Published Online: 22 JUL 2003

DOI: 10.1038/npg.els.0003797

eLS

eLS

How to Cite

Beauchamp, J. 2003. Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane. eLS. .

Author Information

  1. Imperial College School of Medicine, London, UK

Publication History

  1. Published Online: 22 JUL 2003

Abstract

Manifold dot or slot blotting of DNA on to nitrocellulose or uncharged nylon membranes are described together as they involve the same steps but differ in the buffers used and in the method of post-blotting fixation.

Keywords:

  • denaturation;
  • neutralization;
  • immobilization